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  • KD/KO Validated

SPOP Polyclonal antibody

SPOP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 16750-1-AP

Synonyms

TEF2, Speckle-type POZ protein, speckle type POZ protein



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16750-1-AP targets SPOP in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPOP fusion protein Ag10215
Full Name speckle-type POZ protein
Calculated Molecular Weight 374 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC003385
Gene Symbol SPOP
Gene ID (NCBI) 8405
RRIDAB_2756394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The SPOP (TEF2) protein was previously identified as an autoantigen in a patient with scleroderma pigmentosum. SPOP (speckle-type POZ protein), also known as TEF2, HIB homolog 1 or Roadkill homolog 1, is a member of the Tdpoz family containing one N-terminal MATH (Meprin and TRAF Homology) domain and one C-terminal BTB/POZ domain. SPOP can exist as a homodimer and is expressed in a variety of tissues localizing to the nucleus. BTB-mediated SPOP dimers form linear oligomers via BACK domain dimerization, and we determine the concentration-dependent populations of the resulting oligomeric species (PMID: 27220849 ). Through an interaction with CUL-3, SPOP is involved in ubiquitinylation and protein degradation. SPOP specifically interacts with CUL-3 via its BTB/POZ domain and recruits substrates to the CUL-3-based ubiquitin ligase via its MATH domain. Substrates recruited by SPOP and targeted for ubiquitylation via the CUL-3/SPOP complex include PDX-1, Bmi-1, MacroH2A, PIPK II ∫ and Daxx. These substrates are subsequently degraded by the proteasome. In addition, SPOP itself becomes ubiquitylated by the CUL-3-based ubiquitin ligase and is targeted for proteasomal degradation.

Protocols

Product Specific Protocols
WB protocol for SPOP antibody 16750-1-APDownload protocol
IF protocol for SPOP antibody 16750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nature

Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance.

Authors - Jinfang Zhang
  • KO Validated
humanWB

Nat Med

Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4.

Authors - Xiangpeng Dai
  • KO Validated
humanWB

Nat Med

Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation.

Authors - Pingzhao Zhang
  • KD Validated
humanWB

Cell Res

ILF3 is a substrate of SPOP for regulating serine biosynthesis in colorectal cancer.

Authors - Kai Li
  • KD Validated
mouse

Mol Cell

DNA Damage Promotes TMPRSS2-ERG Oncoprotein Destruction and Prostate Cancer Suppression via Signaling Converged by GSK3β and WEE1.

Authors - Zhe Hong
humanWB

Mol Cancer

SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions.

Authors - Qiwei Jiang
  • KD Validated