SPOP Polyclonal antibody

• Featured Product
• KD/KO Validated

SPOP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

78

Cat no : 16750-1-AP

Synonyms

TEF2, Speckle-type POZ protein, speckle type POZ protein

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Validation Data Gallery

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  WB analysis using 16750-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16750-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16750-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16750-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16750-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16750-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16750-1-AP (SPOP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16750-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16750-1-AP (SPOP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 40 publications below
• WB: See 73 publications below
• IHC: See 12 publications below
• IF: See 11 publications below
• IP: See 9 publications below
• CoIP: See 7 publications below
• RIP: See 1 publications below

Product Information

16750-1-AP targets SPOP in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPOP fusion protein Ag10215
• Full Name: speckle-type POZ protein
• Calculated Molecular Weight: 374 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC003385
• Gene Symbol: SPOP
• Gene ID (NCBI): 8405
• RRID: AB_2756394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The SPOP (TEF2) protein was previously identified as an autoantigen in a patient with scleroderma pigmentosum. SPOP (speckle-type POZ protein), also known as TEF2, HIB homolog 1 or Roadkill homolog 1, is a member of the Tdpoz family containing one N-terminal MATH (Meprin and TRAF Homology) domain and one C-terminal BTB/POZ domain. SPOP can exist as a homodimer and is expressed in a variety of tissues localizing to the nucleus. BTB-mediated SPOP dimers form linear oligomers via BACK domain dimerization, and we determine the concentration-dependent populations of the resulting oligomeric species (PMID: 27220849 ). Through an interaction with CUL-3, SPOP is involved in ubiquitinylation and protein degradation. SPOP specifically interacts with CUL-3 via its BTB/POZ domain and recruits substrates to the CUL-3-based ubiquitin ligase via its MATH domain. Substrates recruited by SPOP and targeted for ubiquitylation via the CUL-3/SPOP complex include PDX-1, Bmi-1, MacroH2A, PIPK II ∫ and Daxx. These substrates are subsequently degraded by the proteasome. In addition, SPOP itself becomes ubiquitylated by the CUL-3-based ubiquitin ligase and is targeted for proteasomal degradation.

Publications

Species	Application	Title
human	WB,IP	Nature; Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance.; Authors - Jinfang Zhang ; KO Validated
human	WB	Nat Med; Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4.; Authors - Xiangpeng Dai ; KO Validated
human	WB	Nat Med; Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation.; Authors - Pingzhao Zhang ; KD Validated
human	WB	Cell Res; ILF3 is a substrate of SPOP for regulating serine biosynthesis in colorectal cancer.; Authors - Kai Li ; KD Validated
mouse		Mol Cell; DNA Damage Promotes TMPRSS2-ERG Oncoprotein Destruction and Prostate Cancer Suppression via Signaling Converged by GSK3β and WEE1.; Authors - Zhe Hong
human	WB	Mol Cancer; SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions.; Authors - Qiwei Jiang ; KD Validated

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