SPON2 Monoclonal antibody, PBS Only

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  WB analysis of LNCaP using 66859-1-Ig (same clone as 66859-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66859-1-Ig (SPON2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66859-1-Ig (same clone as 66859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66859-1-Ig (same clone as 66859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

SPON2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66859-1-PBS

Clone No.1H4B11

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

DIL 1, DIL1, M spondin, Mindin, SPON2, Spondin 2

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Product Information

66859-1-PBS targets SPON2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SPON2 fusion protein Ag14337
• Full Name: spondin 2, extracellular matrix protein
• Calculated Molecular Weight: 331 aa, 36 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC002707
• Gene Symbol: SPON2
• Gene ID (NCBI): 10417
• RRID: AB_2882198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BUD6
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SPON2 (Spondin 2), also known as Mindin, is a member of the F-spondin family of secreted extracellular matrix proteins. This protein has been implicated in axon guidance, immune response and adhesion. SPON2 is essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (PMID: 14691481). It also functions as an integrin ligand for inflammatory cell recruitment and T-cell priming (PMID: 19153605). Besides, SPON2 has been reported as a prognostic biomarker for ovarian and prostate cancer (PMID: 17490732; 22615945; 23665271).