SPOCK2 Polyclonal antibody

SPOCK2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11725-1-AP

Synonyms

KIAA0275, SPOCK2, testican 2, TICN2

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Validation Data Gallery

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  IHC staining of human testis using 11725-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11725-1-AP (SPOCK2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 11725-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11725-1-AP (SPOCK2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 11725-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11725-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11725-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11725-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11725-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11725-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11725-1-AP (SPOCK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human testis tissue, human kidney tissue, human pancreas cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

11725-1-AP targets SPOCK2 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPOCK2 fusion protein Ag2313
• Full Name: sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2
• Calculated Molecular Weight: 424 aa, 47 kDa
• GenBank Accession Number: BC023558
• Gene Symbol: SPOCK2
• Gene ID (NCBI): 9806
• RRID: AB_2255261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Diagnostics (Basel); Identification and Validation of T-Cell Exhaustion Signature for Predicting Prognosis and Immune Response in Pancreatic Cancer by Integrated Analysis of Single-Cell and Bulk RNA Sequencing Data; Authors - Yaowu Zhu