SPOCK1 Recombinant antibody

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  WB analysis using 84977-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84977-1-RR (SPOCK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.ce

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  Affinity and Kinetic Characterization of 84977-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84977-1-RR against Human SPOCK1 were performed. The affinity constant is 3.51 nM.

SPOCK1 Recombinant Antibody for WB, ELISA

Cat No. 84977-1-RR

Clone No.242196E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Testican-1, Testican 1, TESTICAN, SPOCK, Protein SPOCK

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Tested Applications

• Positive WB detected in: DU 145 cells, PC-3 eclls, HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84977-1-RR targets SPOCK1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SPOCK1 fusion protein Ag28204
• Full Name: sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
• Calculated Molecular Weight: 439 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC030691
• Gene Symbol: SPOCK1
• Gene ID (NCBI): 6695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q08629
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPOCK1, also known as TICN1. It is predicted to be located in the extracellular matrix. The proein may play a role in cell-cell and cell-matrix interactions and may contribute to various neuronal mechanisms in the central nervous system. The molecular weight of SPOCK1 is 49 kDa.