SPO11 Polyclonal antibody, PBS Only

• 

  WB analysis of mouse testis using 15509-1-AP (same clone as 15509-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15509-1-AP (SPO11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15509-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 15509-1-AP (same clone as 15509-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 15509-1-AP (SPO11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15509-1-PBS in a different storage buffer formulation.

SPO11 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 15509-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

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Product Information

15509-1-PBS targets SPO11 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPO11 fusion protein Ag4340
• Full Name: SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC033591
• Gene Symbol: SPO11
• Gene ID (NCBI): 23626
• RRID: AB_10639508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5K1
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Spo11 is a protein used in a complex along with Mre11 and Rad50 during meiotic recombination. It is also involved in the creation of double stranded breaks in the DNA in the early stages of this process. Several transcript variants encoding different isoforms have been found for this gene.