SPIN1-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

SPIN1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19531-1-AP

Synonyms

SPIN1, spindlin 1, SPIN 1, SPIN, Ovarian cancer-related protein

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Validation Data Gallery

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  WB analysis of SKOV-3 using 19531-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19531-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 19531-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 19531-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 19531-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19531-1-AP

  Immunofluorescent analysis of Hela cells, using SPIN1 antibody 19531-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, A2780 cells, HEK-293 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

19531-1-AP targets SPIN1-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: spindlin 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_006717
• Gene Symbol: SPIN1
• Gene ID (NCBI): 10927
• RRID: AB_10638616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPIN1 (Spindlin 1) is a histone methylation reader, which recognizes trimethylated histone H3 lysine 4 through the protein-protein interaction. SPIN1 was initially found in ovarian cancer via searching ovarian cancer-related genes . It is overexpressed in ovarian cancer tissue, which exhibits a high homology to mouse Spin (Spindlin) gene. Signaling pathways responsible for SPIN1 functions include PI3K/Akt, Wnt and RET that are highly relevant to tumorigenesis.(PMID: 34492335, PMID: 31790762)

Publications

Species	Application	Title
human	WB	Oncotarget; Suppressive role exerted by microRNA-29b-1-5p in triple negative breast cancer through SPIN1 regulation.; Authors - Rosa Drago-Ferrante ; KD Validated
human	WB	J Cell Biochem; Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.; Authors - Sulpizio Marilisa M
human	IHC	Bioengineered; The microRNA-381(miR-381)/Spindlin1(SPIN1) axis contributes to cell proliferation and invasion of colorectal cancer cells by regulating the Wnt/β-catenin pathway.; Authors - Ling Zhou
human	IHC	J Exp Clin Cancer Res; SPIN1, negatively regulated by miR-148/152, enhances Adriamycin resistance via upregulating drug metabolizing enzymes and transporter in breast cancer.; Authors - Xu Chen