SPIB Polyclonal antibody

• 

  WB analysis of L02 using 15768-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15768-1-AP (SPIB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 15768-1-AP

  IP result of anti-SPIB (IP:15768-1-AP, 4ug; Detection:15768-1-AP 1:300) with L02 cells lysate 1800ug.

• 

  IHC staining of human liver cancer using 15768-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15768-1-AP (SPIB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15768-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15768-1-AP (SPIB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPIB Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 15768-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ChIP, ELISA

Synonyms

SPI B, SPIB, Transcription factor Spi B

---

Tested Applications

• Positive WB detected in: L02 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

15768-1-AP targets SPIB in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPIB fusion protein Ag8404
• Full Name: Spi-B transcription factor (Spi-1/PU.1 related)
• Calculated Molecular Weight: 262 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC007921
• Gene Symbol: SPIB
• Gene ID (NCBI): 6689
• RRID: AB_2878182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01892
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Signal; SPIB acts as a tumor suppressor by activating the NFkB and JNK signaling pathways through MAP4K1 in colorectal cancer cells.; Authors - Xunping Zhao
human	WB,IHC,ChIP	J Exp Clin Cancer Res; tsRNA-GlyGCC promotes colorectal cancer progression and 5-FU resistance by regulating SPIB; Authors - Rong Xu