SPHK1 Recombinant antibody, PBS Only

SPHK1 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241017A6

Cat no : 84640-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of THP-1 using 84640-1-RR (same clone as 84640-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84640-1-RR (SPHK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84640-1-PBS in a different storage buffer formulation.

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Product Information

84640-1-PBS targets SPHK1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: sphingosine kinase 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_182965
• Gene Symbol: SPHK1
• Gene ID (NCBI): 8877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).