SPHK1 Recombinant antibody

SPHK1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82609-1-RR
Clone No.2K12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Sphingosine kinase 1, SK1, EC:2.7.1.91, EC:2.3.1.-, Acetyltransferase SPHK1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHepG2 cells, L02 cells, MCF-7 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82609-1-RR targets SPHK1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SPHK1 fusion protein Ag1051 Predict reactive species
Full Name sphingosine kinase 1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC008040
Gene Symbol SPHK1
Gene ID (NCBI) 8877
RRIDAB_3086491
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NYA1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).

Protocols

Product Specific Protocols
WB protocol for SPHK1 antibody 82609-1-RRDownload protocol
IF protocol for SPHK1 antibody 82609-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...