SPG7 Polyclonal antibody

SPG7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27801-1-AP

Synonyms

CAR, CMAR, Paraplegin, PGN, Spastic paraplegia 7 protein, SPG5C, SPG7

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Validation Data Gallery

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  WB analysis using 27801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27801-1-AP (SPG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 27801-1-AP

  IP result of anti-SPG7 (IP:27801-1-AP, 4ug; Detection:27801-1-AP 1:500) with HeLa cells lysate 2800 ug.

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  IHC staining of human liver using 27801-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27801-1-AP (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 27801-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27801-1-AP (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, LNCaP cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27801-1-AP targets SPG7 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPG7 fusion protein Ag26265
• Full Name: spastic paraplegia 7 (pure and complicated autosomal recessive)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 54 kDa, 70 kDa, 80kDa
• GenBank Accession Number: BC007692
• Gene Symbol: SPG7
• Gene ID (NCBI): 6687
• RRID: AB_2918133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPG7 encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA protein family. Two transcript variants encoding distinct isoforms have been identified. The molecular weight of SPG7 is about 88-100 kDa (precursor),80 kDa (intermediate) and 70 kDa (mature)(PMID: 24767997), while the molecular weight of isoform 2 is about 54 kDa.