SPEG Polyclonal antibody, PBS Only

SPEG Polyclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18716-1-PBS

Synonyms

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Validation Data Gallery

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  IHC staining of mouse heart using 18716-1-AP (same clone as 18716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18716-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 18716-1-AP (same clone as 18716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18716-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 18716-1-AP (same clone as 18716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18716-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 18716-1-AP (same clone as 18716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18716-1-PBS in a different storage buffer formulation.

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Product Information

18716-1-PBS targets SPEG in IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SPEG complex locus
• Calculated Molecular Weight: 354 kDa
• GenBank Accession Number: NM_005876
• Gene Symbol: SPEG
• Gene ID (NCBI): 10290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SPEG, also named as BPEG, APEG1 and KIAA1297, is highly similar to the corresponding rat and mouse proteins. Expression of SPEG is thought to serve as a marker for differentiated vascular smooth muscle cells which may have a role in regulating growth and differentiation of this cell type. SPEG is expressed in vascular smooth muscle cells and is down-regulated by vascular injury. Multiple transcripts are produced from the SPEG complex locus by alternative splicing and alternative promoter usage. The antibody recognizes the C-term of SPEG.