SPATS2L Polyclonal antibody

• Featured Product
• KD/KO Validated

SPATS2L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16938-1-AP

Synonyms

LOC26010, SPATS2 like protein, SP1224, SGNP, DNAPTP6

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Validation Data Gallery

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  WB analysis of HepG2 using 16938-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16938-1-AP (SPATS2L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16938-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16938-1-AP (SPATS2L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16938-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16938-1-AP (SPATS2L antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16938-1-AP

  Immunofluorescent analysis of HepG2 cells, using LOC26010 antibody 16938-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of Saos-2 using 16938-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using SPATS2L antibody (16938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human heart tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Saos-2 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16938-1-AP targets SPATS2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPATS2L fusion protein Ag10438
• Full Name: viral DNA polymerase-transactivated protein 6
• Calculated Molecular Weight: 488 aa, 54 kDa
• Observed Molecular Weight: 54-62 kDa
• GenBank Accession Number: BC018736
• Gene Symbol: SPATS2L
• Gene ID (NCBI): 26010
• RRID: AB_1851075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPATS2L encodes spermatogenesis associated and serine-rich 2-like protein, also named DNAPTP6 (DNA polymerase transactivated protein 6) in human (PMID:11230166). SPATS2L acts as an important regulator of β2-adrenergic receptor down-regulation (PubMed: 25562107). MiR-1269a down-regulates the expression of SPATS2L and LRP6, thereby inhibiting the proliferation of liver cancer cells (PMID: 28081866, PMID:35252180). SPATS2L has several isoforms by alternative splicing.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu
human	IHC,WB	Sci Rep; Characteristic DNA methylation profiles of chorionic villi in recurrent miscarriage.; Authors - Yosuke Matsumoto ; KD Validated
human	WB	J Transl Med; Spermatogenesis associated serine rich 2 like plays a prognostic factor and therapeutic target in acute myeloid leukemia by regulating the JAK2/STAT3/STAT5 axis; Authors - Fenglin Li ; KD Validated
mouse	IF	Sci Adv; RNA G-quadruplex organizes stress granule assembly through DNAPTP6 in neurons; Authors - Sefan Asamitsu