SPATA7 Polyclonal antibody

• Featured Product
• KD/KO Validated

SPATA7 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12020-1-AP

Synonyms

HSD 3.1, HSD3, SPATA7, spermatogenesis associated 7

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Validation Data Gallery

• 

  WB analysis using 12020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12020-1-AP (SPATA7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12020-1-AP

  IP result of anti-SPATA7 (IP:12020-1-AP, 3ug; Detection:12020-1-AP 1:300) with mouse testis tissue lysate 8000ug.

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12020-1-AP targets SPATA7 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPATA7 fusion protein Ag2649
• Full Name: spermatogenesis associated 7
• Calculated Molecular Weight: 599 aa, 68 kDa
• Observed Molecular Weight: 68-80 kDa
• GenBank Accession Number: BC008656
• Gene Symbol: SPATA7
• Gene ID (NCBI): 55812
• RRID: AB_2195380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPATA7, also named as HSD3, may be involved in retinal function. It is interesting to note that mutations in SPATA7 cause LCA and RP/ARRP , two overlapping but distinct human retinal diseases. SPATA7 is another LCA and Juvenile RP Gene.(PMID:19268277) It is a highly conserved, vertebrate-specific protein which expressed in the mature retina. For some modification, the MW always migrate 5-8kd. The antibody can reoginze all the 3 isoforms of SPATA7.

Publications

Species	Application	Title
mouse	IF, IHC	Am J Hum Genet; Mutations in SPATA7 cause Leber congenital amaurosis and juvenile retinitis pigmentosa.; Authors - Wang Hui H
mouse	WB	Hum Mol Genet; Spata7 is a retinal ciliopathy gene critical for correct RPGRIP1 localization and protein trafficking in the retina.; Authors - Aiden Eblimit ; KO Validated
mouse	WB,IF	Gene Ther; AAV8(Y733F)-mediated gene therapy in a Spata7 knockout mouse model of Leber congenital amaurosis and retinitis pigmentosa.; Authors - H Zhong ; KO Validated
mouse	WB	Exp Eye Res; Conditional loss of Spata7 in photoreceptors causes progressive retinal degeneration in mice.; Authors - Aiden Eblimit

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