SPATA2 Polyclonal antibody
SPATA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 29321-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue, rat testis tissue |
Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
29321-1-AP targets SPATA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SPATA2 fusion protein Ag30773 |
Full Name | spermatogenesis associated 2 |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 58-60 kDa |
GenBank Accession Number | BC009481 |
Gene Symbol | SPATA2 |
Gene ID (NCBI) | 9825 |
RRID | AB_2918282 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The protein SPATA2 (spermatogenesis-associated protein 2) was initially shown to be highly expressed in testicular Sertoli cells, and these early reports suggested SPATA2 to play a role in spermatogenesis. More recently, it was discussed to be involved in the predisposition for psoriasis. SPATA2 is required for TNF-induced cell death. SPATA2 has a potential role during pancreatic development and β-cell proliferation.(PMID: 20119533/PMID: 27458237)
Protocols
Product Specific Protocols | |
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WB protocol for SPATA2 antibody 29321-1-AP | Download protocol |
IHC protocol for SPATA2 antibody 29321-1-AP | Download protocol |
IF protocol for SPATA2 antibody 29321-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |