SPARCL1 Monoclonal antibody

SPARCL1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A9E7

Cat no : 67511-1-Ig

Synonyms

Hevin, MAST 9, PIG33, SC1, SPARC like 1 (hevin), SPARC like protein 1, SPARCL1

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Validation Data Gallery

• 

  WB analysis of human saliva using 67511-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 67511-1-Ig (SPARCL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 67511-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 67511-1-Ig (SPARCL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human saliva, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67511-1-Ig targets SPARCL1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SPARCL1 fusion protein Ag29092
• Full Name: SPARC-like 1 (hevin)
• Calculated Molecular Weight: 664 aa, 75 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC033721
• Gene Symbol: SPARCL1
• Gene ID (NCBI): 8404
• RRID: AB_2882732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.