SPARC Monoclonal antibody, PBS Only

SPARC Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A2C1

Cat no : 66426-1-PBS

Synonyms

Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC

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Validation Data Gallery

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  WB analysis of A375 using 66426-1-Ig (same clone as 66426-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66426-1-Ig (same clone as 66426-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66426-1-Ig (same clone as 66426-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66426-1-Ig (same clone as 66426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66426-1-Ig (SPARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66426-1-Ig (same clone as 66426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66426-1-Ig (SPARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  IF Staining of C6 using 66426-1-Ig (same clone as 66426-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using SPARC antibody (66426-1-Ig, Clone: 1A2C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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Product Information

66426-1-PBS targets SPARC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SPARC fusion protein Ag7390
• Full Name: secreted protein, acidic, cysteine-rich (osteonectin)
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 38 kDa, 52 kDa
• GenBank Accession Number: BC004974
• Gene Symbol: SPARC
• Gene ID (NCBI): 6678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).