SPAG9 Polyclonal antibody

SPAG9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24423-1-AP

Synonyms

Cancer/testis antigen 89, CT89, HLC 6, HLC4, HSS, JIP 4, JLP, JNK interacting protein 4, KIAA0516, MAPK8IP4, PHET, PIG6, SPAG9, sperm associated antigen 9, Sperm specific protein, Sperm surface protein, Sunday driver 1, SYD1

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Validation Data Gallery

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  WB analysis using 24423-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24423-1-AP (SPAG9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24423-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24423-1-AP (SPAG9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 24423-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24423-1-AP (SPAG9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 24423-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24423-1-AP (SPAG9 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 24423-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24423-1-AP (SPAG9 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of RAW 264.7 using 24423-1-AP

  Immunofluorescent analysis of RAW 264.7 cells using 24423-1-AP (SPAG9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, RAW 264.7 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24423-1-AP targets SPAG9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPAG9 fusion protein Ag19763
• Full Name: sperm associated antigen 9
• Calculated Molecular Weight: 1321 aa, 146 kDa
• Observed Molecular Weight: 146 kDa
• GenBank Accession Number: BC146755
• Gene Symbol: SPAG9
• Gene ID (NCBI): 9043
• RRID: AB_2879541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.