SPAG7 Polyclonal antibody, PBS Only

SPAG7 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16294-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse ovary using 16294-1-AP (same clone as 16294-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16294-1-AP (same clone as 16294-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 16294-1-AP (same clone as 16294-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 16294-1-AP (same clone as 16294-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16294-1-AP (SPAG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 16294-1-AP (same clone as 16294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 16294-1-AP (same clone as 16294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16294-1-AP (SPAG7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16294-1-PBS in a different storage buffer formulation.

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Product Information

16294-1-PBS targets SPAG7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPAG7 fusion protein Ag9471
• Full Name: sperm associated antigen 7
• Calculated Molecular Weight: 227 aa, 26 kDa
• Observed Molecular Weight: 26-31 kDa
• GenBank Accession Number: BC011934
• Gene Symbol: SPAG7
• Gene ID (NCBI): 9552
• RRID: AB_2302478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SPAG7 encodes the sperm-associated antigen 7, which is widely expressed. The SPAG7 protein contains two nuclear localisation signals and a R3H domain. The domain is predicted to bind ssDNA or ssRNA in a sequence-specific manner. This might indicate a potential role of the protein in response to viruses or free DNAs or RNAs.