SPAG4L Polyclonal antibody

• Featured Product
• KD/KO Validated

SPAG4L Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 17495-1-AP

Synonyms

SPAG4L, SUN5, TSARG4

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Validation Data Gallery

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  WB analysis using 17495-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17495-1-AP (SPAG4L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 17495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using 17495-1-AP (SPAG4L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 12 publications below

Product Information

17495-1-AP targets SPAG4L in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPAG4L fusion protein Ag11629
• Full Name: sperm associated antigen 4-like
• Calculated Molecular Weight: 379 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC026118
• Gene Symbol: SPAG4L
• Gene ID (NCBI): 140732
• RRID: AB_1939754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPAG4L,also named as SUN domain-containing protein 5 or Testis and spermatogenesis-related gene 4 protein, is a 379 amino acid protein, which contains 1 SUN domain. SPAG4L is widely expressed and localizes in the nucleus inner membrane. SPAG4L is restricted to the apical nuclear region of round spermatids that face the acrosomic vesicle. SPAG4L is involved in linkage of the acrosomic vesicle to the spermatid nucleus, and in acrosome biogenesis.

Publications

Species	Application	Title
mouse	IF	Nat Commun; The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.; Authors - Ying Zhang
human	WB	Am J Hum Genet; Biallelic SUN5 Mutations Cause Autosomal-Recessive Acephalic Spermatozoa Syndrome.; Authors - Fuxi Zhu
human	IF	Am J Hum Genet; Mutations in PMFBP1 Cause Acephalic Spermatozoa Syndrome.; Authors - Fuxi Zhu
mouse	WB,IF	Elife; Essential role for SUN5 in anchoring sperm head to the tail.; Authors - Yongliang Shang ; KO Validated
human	IF	Int J Mol Sci; Identification and Characterization of the Most Common Genetic Variant Responsible for Acephalic Spermatozoa Syndrome in Men Originating from North Africa.; Authors - Caroline Cazin
human	WB,IF	Cancers (Basel); Nuclear Membrane Protein SUN5 Is Highly Expressed and Promotes Proliferation and Migration in Colorectal Cancer by Regulating the ERK Pathway; Authors - Xiaoyue Song