SP140L Polyclonal antibody

SP140L Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 19845-1-AP

Synonyms

SP140L

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Validation Data Gallery

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  WB analysis of HeLa using 19845-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19845-1-AP (SP140L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19845-1-AP

  IP result of anti-SP140L (IP:19845-1-AP, 4ug; Detection:19845-1-AP 1:500) with HeLa cells lysate 3200ug.

• 

  IP experiment of HeLa using 19845-1-AP

  IP result of anti-SP140L (IP:19845-1-AP, 4ug; Detection:19845-1-AP 1:500) with HeLa cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19845-1-AP is SP140L Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SP140L fusion protein Ag13894
• Full Name: SP140 nuclear body protein-like
• Calculated Molecular Weight: 438 aa, 50 kDa
• Observed Molecular Weight: 67-77 kDa
• GenBank Accession Number: BC004921
• Gene Symbol: SP140L
• Gene ID (NCBI): 93349
• RRID: AB_2878613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.