SP100 Recombinant antibody, PBS Only (Detector)

SP100 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241153B4

Cat no : 84012-1-PBS

Synonyms

Speckled 100 kDa, SP100 nuclear antigen, SP 100, Nuclear dot-associated Sp100 protein, Nuclear autoantigen Sp-100

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00964-4

  Sandwich ELISA standard curve of MP00964-4, Human SP100 Recombinant Matched Antibody Pair - PBS only. 84012-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35193. 84012-1-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

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  Sample test of MP00964-4

  The mean SP100 concentration was determined to be 922.7 pg/mL in A431 cell extract based on a 1.5 mg/mL extract load.

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  Cytometric bead array standard curve of MP00964-3

  Cytometric bead array standard curve of MP00964-3, SP100 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84012-2-PBS. Detection antibody: 84012-1-PBS. Standard: Ag35193. Range: 0.156-10 ng/mL.

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Product Information

84012-1-PBS targets SP100 as part of a matched antibody pair:

MP00964-3: 84012-2-PBS capture and 84012-1-PBS detection (validated in Cytometric bead array)

MP00964-4: 84012-6-PBS capture and 84012-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SP100 fusion protein Ag35193
• Full Name: SP100 nuclear antigen
• Calculated Molecular Weight: 879 aa, 100 kDa
• GenBank Accession Number: BC011562
• Gene Symbol: SP100
• Gene ID (NCBI): 6672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.