SP100 Polyclonal antibody, PBS Only

• 

  WB analysis of Jurkat using 11377-1-AP (same clone as 11377-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11377-1-AP (SP100 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11377-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11377-1-AP (same clone as 11377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11377-1-AP (SP100 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11377-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11377-1-AP (same clone as 11377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11377-1-AP (SP100 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11377-1-PBS in a different storage buffer formulation.

SP100 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 11377-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

---

Product Information

11377-1-PBS targets SP100 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SP100 fusion protein Ag1939
• Full Name: SP100 nuclear antigen
• Calculated Molecular Weight: 879 aa, 100 kDa
• Observed Molecular Weight: 70-100 kDa, 110-130 kDa
• GenBank Accession Number: BC011562
• Gene Symbol: SP100
• Gene ID (NCBI): 6672
• RRID: AB_2194302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23497
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.