SOX8 Polyclonal antibody

• Featured Product
• KD/KO Validated

SOX8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 20627-1-AP

Synonyms

SOX8, Transcription factor SOX 8

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Validation Data Gallery

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  WB analysis using 20627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20627-1-AP (SOX8 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 20627-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20627-1-AP (SOX8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 20627-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20627-1-AP (SOX8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20627-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20627-1-AP (SOX8 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20627-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20627-1-AP (SOX8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, COLO 320 cells, rat brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

20627-1-AP targets SOX8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOX8 fusion protein Ag14556
• Full Name: SRY (sex determining region Y)-box 8
• Calculated Molecular Weight: 446 aa, 47 kDa
• Observed Molecular Weight: 47-55 kDa
• GenBank Accession Number: BC031797
• Gene Symbol: SOX8
• Gene ID (NCBI): 30812
• RRID: AB_10693538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Cell; Thymic epithelial cells co-opt lineage-defining transcription factors to eliminate autoreactive T cells.; Authors - Daniel A Michelson
human	IHC	Cancer Biol Ther; The oncogenic role of SOX8 in endometrial carcinoma.; Authors - Wenyan Tian
human	WB,IHC	Carcinogenesis; SOX8 acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer.; Authors - Hailin Tang
human	WB,IHC	Redox Biol; Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis; Authors - Xue Yang ; KO Validated