SOX21 Polyclonal antibody

SOX21 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55052-1-AP

Synonyms

SOX A, SOX21, SOX25, SOXA, Transcription factor SOX 21

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Validation Data Gallery

• 

  WB analysis of mouse embryo using 55052-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 55052-1-AP (SOX21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55052-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55052-1-AP (SOX21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 55052-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 55052-1-AP (SOX21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55052-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55052-1-AP (SOX21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse embryo tissue, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

55052-1-AP targets SOX21 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SRY (sex determining region Y)-box 21
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: NM_007084
• Gene Symbol: SOX21
• Gene ID (NCBI): 11166
• RRID: AB_2881260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Res; New mechanisms of resistance to MEK inhibitors in melanoma revealed by intravital imaging.; Authors - Hailey E Brighton
goat	WB	Animals (Basel); Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications.; Authors - Sen Ma
human	WB	Life Sci; Intervention of epithelial mesenchymal transition against colon cancer cell growth and metastasis based on SOX21/POU4F2/Hedgehog signaling axis; Authors - Qiaochang Cao