CoraLite® Plus 488-conjugated SOX2 Recombinant antibody

SOX2 Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230225G1

Cat no : CL488-83059-5

Synonyms

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

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Validation Data Gallery

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  IF Staining of U-251 using CL488-83059-5

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite® Plus 488 SOX2 antibody (CL488-83059-5, Clone: 230225G1 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83059-5 targets SOX2 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SOX2 fusion protein Ag0612
• Full Name: SRY (sex determining region Y)-box 2
• Calculated Molecular Weight: 34 kDa
• GenBank Accession Number: BC013923
• Gene Symbol: SOX2
• Gene ID (NCBI): 6657
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. Affinity purified rabbit anti-Sox2 antibody can be used to demonstrate pluripotency of ES and iPS cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human SOX2. a rare undiferentiated cell population that is intermingled with the Bergmann glia of the adult murine cerebellar cortex, expresses the stem cell markers Sox2 and Nestin, and lacks markers of glial or neuronal diferentiation. Sox2-expressing neural stem cells in the subgranular zone (SGZ) , a well-known stem cell niche of the adult brain.