SOX2 Recombinant antibody

SOX2 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230225G1

Cat no : 83059-5-RR

Synonyms

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

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Validation Data Gallery

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  IF Staining of U-251 using 83059-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (83059-5-RR, Clone: 230225G1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).CL594-Phalloidin (red).

• 

  IF Staining of U-251 using 83059-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (83059-5-RR, Clone: 230225G1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  Affinity and Kinetic Characterization of 83059-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83059-5-RR against Human SOX2 were performed. The affinity constant is 3.54 nM.

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Tested Applications

• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83059-5-RR targets SOX2 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SOX2 fusion protein Ag0612
• Full Name: SRY (sex determining region Y)-box 2
• Calculated Molecular Weight: 34 kDa
• GenBank Accession Number: BC013923
• Gene Symbol: SOX2
• Gene ID (NCBI): 6657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. Affinity purified rabbit anti-Sox2 antibody can be used to demonstrate pluripotency of ES and iPS cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human SOX2. a rare undiferentiated cell population that is intermingled with the Bergmann glia of the adult murine cerebellar cortex, expresses the stem cell markers Sox2 and Nestin, and lacks markers of glial or neuronal diferentiation. Sox2-expressing neural stem cells in the subgranular zone (SGZ) , a well-known stem cell niche of the adult brain.