SOST/Sclerostin Polyclonal antibody

• Featured Product
• KD/KO Validated

SOST/Sclerostin Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 21933-1-AP

Synonyms

SOST, VBCH, UNQ2976/PRO7455/PRO7476, SOST1, Sclerostin

---

Validation Data Gallery

• 

  IHC staining of human kidney using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21933-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Sclerostin antibody (21933-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Tested Applications

• Positive IHC detected in: human kidney tissue, human breast cancer tissue, mouse lung tissue, mouse embryo tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 8 publications below
• IF: See 3 publications below

Product Information

21933-1-AP targets SOST/Sclerostin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOST/Sclerostin fusion protein Ag16567
• Full Name: sclerosteosis
• Calculated Molecular Weight: 213 aa, 24 kDa
• GenBank Accession Number: BC101086
• Gene Symbol: Sclerostin
• Gene ID (NCBI): 50964
• RRID: AB_2878947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease

Publications

Species	Application	Title
mouse	IF	EMBO Mol Med; Sigma-1 receptor attenuates osteoclastogenesis by promoting ER-associated degradation of SERCA2.; Authors - Xiaoan Wei
rat	WB	Aging (Albany NY); Cyclooxygenase-2/sclerostin mediates TGF-β1-induced calcification in vascular smooth muscle cells and rats undergoing renal failure.; Authors - Fang He
human	WB,IHC	Front Oncol; Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6.; Authors - Hanqing Wang ; KD Validated
mouse	IHC	Ann Hematol; Higher Sclerostin/SOST expression is associated with lower percentage of circulatory blasts and better prognosis in patients with myelofibrosis.; Authors - Marko Lucijanic
rat	IHC	Clin Oral Investig; Mechanisms of sphingosine-1-phosphate (S1P) signaling on excessive stress-induced root resorption during orthodontic molar intrusion.; Authors - Han Wang
mouse	WB	J Orthop Surg Res; Lysyl oxidase inhibits BMP9-induced osteoblastic differentiation through reducing Wnt/β-catenin via HIF-1a repression in 3T3-L1 cells; Authors - Jie Zhang

Related Products

LRP5 Polyclonal antibody

24899-1-AP

DKK1 Polyclonal antibody

21112-1-AP

PTH-Specific Polyclonal antibody

19001-1-AP

RUNX2 Polyclonal antibody

20700-1-AP

ErbB3/HER3 Polyclonal antibody

10369-1-AP

BMP7 Polyclonal antibody

12221-1-AP

CYR61/CCN1 Polyclonal antibody

26689-1-AP