SORLA Polyclonal antibody

• Featured Product
• KD/KO Validated

SORLA Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 22592-1-AP

Synonyms

SORL1, SorLA 1, SORL 1, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, LDLR relative with 11 ligand-binding repeats

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Validation Data Gallery

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  WB analysis of mouse brain using 22592-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22592-1-AP (SORLA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22592-1-AP

  IP result of anti-SORLA (IP:22592-1-AP, 5ug; Detection:22592-1-AP 1:500) with mouse brain tissue lysate 10000ug.

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  IHC staining of mouse brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22592-1-AP (SORLA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22592-1-AP (SORLA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22592-1-AP (SORLA antibody at dilution of 1:50.

• 

  IHC staining of human brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22592-1-AP (SORLA antibody at dilution of 1:50.

• 

  IHC staining of rat brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22592-1-AP (SORLA antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat brain using 22592-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22592-1-AP (SORLA antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse cerebellum using 22592-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using SORLA antibody (22592-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

22592-1-AP targets SORLA in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SORLA fusion protein Ag18445
• Full Name: sortilin-related receptor, L(DLR class) A repeats-containing
• Calculated Molecular Weight: 2214 aa, 248 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: BC137171
• Gene Symbol: SORLA
• Gene ID (NCBI): 6653
• RRID: AB_2879131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SorLA also known as SorL1 or LR11, is a multiligand receptor belonging to the LDL receptor superfamily. It is also a member of vacuolar protein sorting-10 (Vps10) family and is enriched in the brain. SorLA acts as an sorting receptor for APP and prevents amyloidogenic processing of APP. Downregulation of SorLA has been found in patients with sporadic Alzheimer disease (AD). Currently SorLA has emerged as a potential target for AD therapy. (25404298)

Publications

Species	Application	Title
mouse	WB, IP	Proc Natl Acad Sci U S A; α2A adrenergic receptor promotes amyloidogenesis through disrupting APP-SorLA interaction.; Authors - Yunjia Chen
mouse	WB	Environ Res; Exposure to a real traffic environment impairs brain cognition in aged mice; Authors - Chenlu Xu
human	WB,IHC,IF,CoIP	Funct Integr Genomics; SORL1 stabilizes ABCB1 to promote cisplatin resistance in ovarian cancer; Authors - Zhen Zheng ; KD Validated
rat	WB,IF	Adv Sci (Weinh); Endo-Lysosomal Network Disorder Reprograms Energy Metabolism in SorL1-Null Rat Hippocampus; Authors - Yajie Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ping (Verified Customer) (06-04-2019)

This antibody works well for immunoprecipitation and western blot.

• Applications: Western Blot, Immunoprecipitation,
• Cell Tissue Type: mouse brain tissue