SORD Monoclonal antibody, PBS Only

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  WB analysis using 67625-1-Ig (same clone as 67625-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67625-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67625-1-Ig (same clone as 67625-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67625-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67625-1-Ig (same clone as 67625-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67625-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67625-1-Ig (same clone as 67625-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67625-1-PBS in a different storage buffer formulation.

SORD Monoclonal Antibody for WB, ELISA

Cat No. 67625-1-PBS

Clone No.4A8E1

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, ELISA

Synonyms

L iditol 2 dehydrogenase, SDH, sorbitol dehydrogenase, SORD, SORD1

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67625-1-Ig

Product Information

67625-1-PBS targets SORD in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SORD fusion protein Ag8651
• Full Name: sorbitol dehydrogenase
• Calculated Molecular Weight: 357 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC021085
• Gene Symbol: SORD
• Gene ID (NCBI): 6652
• RRID: AB_2882826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q00796
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

SORD(Sorbitol dehydrogenase) is also named as L-iditol 2-dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. It catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. This protein can form a homotetramer(PMID:12962626). Measurement of SORD is a specific indicator of liver cell damage and parenchymal.