Sorbitol dehydrogenase Polyclonal antibody

• Featured Product
• KD/KO Validated

Sorbitol dehydrogenase Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15881-1-AP

Synonyms

SORD, EC:1.1.1.9, EC:1.1.1.56, EC:1.1.1.4, EC:1.1.1.14

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Validation Data Gallery

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  WB analysis of HeLa using 15881-1-AP

  WB result of Sorbitol dehydrogenase antibody (15881-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Sorbitol dehydrogenase transfected HeLa cells.

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  WB analysis using 15881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15881-1-AP (Sorbitol dehydrogenase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15881-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15881-1-AP (Sorbitol dehydrogenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15881-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15881-1-AP (Sorbitol dehydrogenase antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15881-1-AP

  IP result of anti-Sorbitol dehydrogenase (IP:15881-1-AP, 4ug; Detection:15881-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human ovary cancer using 15881-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15881-1-AP (Sorbitol dehydrogenase antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human kidney tissue, rat liver tissue, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

15881-1-AP targets Sorbitol dehydrogenase in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Sorbitol dehydrogenase fusion protein Ag8651
• Full Name: sorbitol dehydrogenase
• Calculated Molecular Weight: 357 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC021085
• Gene Symbol: SORD
• Gene ID (NCBI): 6652
• RRID: AB_2192275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SORD(Sorbitol dehydrogenase) is also named as L-iditol 2-dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. It catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. This protein can form a homotetramer(PMID:12962626). Measurement of SORD is a specific indicator of liver cell damage and parenchymal.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Endogenous fructose production and metabolism in the liver contributes to the development of metabolic syndrome.; Authors - Lanaspa Miguel A MA
mouse	WB,IF	Reproduction; Nicotine reduces Sord expression and tyrosine phosphorylation in capacitated sperm.; Authors - Jingbo Dai
mouse	WB	J Mol Med (Berl); Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.; Authors - Yu Sun
rat	WB	Chem Biol Interact; Hepatocyte ferroptosis contributes to anti-tuberculosis drug-induced liver injury: Involvement of the HIF-1α/SLC7A11/GPx4 axis; Authors - Yining Liu
mouse	WB	Nutrients; Endogenous Fructose Production and Metabolism Drive Metabolic Dysregulation and Liver Disease in Mice with Hereditary Fructose Intolerance; Authors - Ana Andres-Hernando
mouse	WB	J Hepatol; Ketohexokinase-C regulates global protein acetylation to decrease carnitine palmitoyltransferase 1a-mediated fatty acid oxidation; Authors - Robert N Helsley