SORCS1 Polyclonal antibody

SORCS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23002-1-AP

Synonyms

hSorCS, SORCS, SORCS1

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Validation Data Gallery

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  WB analysis using 23002-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23002-1-AP (SORCS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 23002-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23002-1-AP (SORCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 23002-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 23002-1-AP (SORCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 23002-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23002-1-AP (SORCS1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 23002-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23002-1-AP (SORCS1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, human brain tissue, Y79 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

23002-1-AP targets SORCS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SORCS1 fusion protein Ag19199
• Full Name: sortilin-related VPS10 domain containing receptor 1
• Calculated Molecular Weight: 1168 aa, 130 kDa
• Observed Molecular Weight: 60-80 kDa
• GenBank Accession Number: BC131597
• Gene Symbol: SORCS1
• Gene ID (NCBI): 114815
• RRID: AB_2879196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SORCS1, also named as SorCS, belongs to the family of VPS10 domain-containing receptors. SorCS1 immunoreactivity is widespread in a population of neurons throughout the brain. Two different types of cellular localization were observed. Most SorCS1 immunoreactive neurons exhibit a punctate cytoplasmic staining which extends into the dendrites, whilst occassionally SorCS1 neuronal immunoreactivity is associated with the plasma membrane.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); CircSPIDR acts as a tumour suppressor in cervical adenocarcinoma by sponging miR-431-5p and regulating SORCS1 and CUBN expression.; Authors - Junfen Xu