SORCS1 Polyclonal antibody

SORCS1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18839-1-AP

Synonyms

hSorCS, SORCS1

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Validation Data Gallery

• 

  WB analysis of Y79 using 18839-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18839-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 18839-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Y79 cells, HEK-293 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18839-1-AP targets SORCS1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: sortilin-related VPS10 domain containing receptor 1
• Calculated Molecular Weight: 1168 aa, 130 kDa
• Observed Molecular Weight: 130 kDa, 60-80 kDa
• GenBank Accession Number: BC131597
• Gene Symbol: SORCS1
• Gene ID (NCBI): 114815
• RRID: AB_2878564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SorCS1 is a 130 kDa type I transmembrane protein, belonging to Vps10p-domain (Vps10p-D) receptor family that comprises an N-terminal Vps10p-D, a leucine-rich domain, a single transmembrane domain, and a short cytoplasmic domain. SorCS1 is synthesized as a proprotein, which is converted to the mature receptor in late Golgi compartments by cleavage of the N-terminal propeptide. Alternative splicing of human SorCS1 results in four isoforms with different cytoplasmic domains and differential expression in tissues. This antibody was generated against the C-terminal region of human SorCS1 and recognizes various forms of SorCS1. Multiple bands around 100-130 kDa as well as 60-80 kDa have been detected in Y79 cells by western blot assay.