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SORBS2 Polyclonal antibody

SORBS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24643-1-AP

Synonyms

Arg/Abl interacting protein 2, ARGBP2, KIAA0777, nArgBP2, PRO0618, Sorbin, SORBS2



Tested Applications

Positive WB detected inA2780 cells, MDCK cells, U-251 cells, mouse pancreas tissue, rat pancreas tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24643-1-AP targets SORBS2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Reactivity human, mouse, canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SORBS2 fusion protein Ag20041
Full Name sorbin and SH3 domain containing 2
Calculated Molecular Weight 1100 aa, 124 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC011883
Gene Symbol SORBS2
Gene ID (NCBI) 8470
RRIDAB_2879652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).

Protocols

Product Specific Protocols
WB protocol for SORBS2 antibody 24643-1-APDownload protocol
IHC protocol for SORBS2 antibody 24643-1-APDownload protocol
IP protocol for SORBS2 antibody 24643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
humanWB,IHC,IF,IP

EBioMedicine

Elevated myocardial SORBS2 and the underlying implications in left ventricular noncompaction cardiomyopathy.

Authors - Chunyan Li
human,mouseWB,IP

Aging (Albany NY)

The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.

Authors - Chunyan Li
humanWB, IP

J Biol Chem

Arg Kinase Binding Protein 2 (ArgBP2) interaction with α-actinin and actin stress fibres inhibits cell migration.

Authors - Praju Vikas Anekal
humanWB

J Transl Med

SORBS2 as a molecular target for atherosclerosis in patients with familial hypercholesterolemia.

Authors - Ming-Ming Liu
humanWB

Mol Med Rep

Musk ketone induces apoptosis of gastric cancer cells via downregulation of sorbin and SH3 domain containing 2.

Authors - Juan An
  • KD Validated