Various lysates were subjected to SDS PAGE followed by western blot with 83519-4-RR (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 83519-4-RR (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary cancer using 83519-4-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 83519-4-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HUVEC using 83519-4-RR
Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (83519-4-RR, Clone: 240462H8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (83519-4-RR, Clone: 240462H8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of HL-60 using 83519-4-RR
1x10^6 HL-60 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HL-60 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of A549 using 83519-4-RR
1x10^6 A549 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 A549 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 83519-4-RR
Biolayer interferometry (BLl) kinetic assays of 83519-4-RR
against Human SOD2 were performed. The affinity constant is 219 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HepG2 cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected in
human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HUVEC cells
Positive FC (Intra) detected in
A549 cells, HL-60 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
83519-4-RR targets SOD2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.
Various lysates were subjected to SDS PAGE followed by western blot with 83519-4-RR (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary cancer using 83519-4-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 83519-4-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HUVEC using 83519-4-RR
Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (83519-4-RR, Clone: 240462H8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC (INTRA) Figures
FC experiment of HL-60 using 83519-4-RR
1x10^6 HL-60 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of A549 using 83519-4-RR
1x10^6 A549 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 83519-4-RR
Biolayer interferometry (BLl) kinetic assays of 83519-4-RR
against Human SOD2 were performed. The affinity constant is 219 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
SOD2 Recombinant antibody
Catalog Number
83519-4-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:1500-1:6000 IF/ICC : 1:400-1:1600 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
