SOD2 Recombinant antibody

SOD2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240462H8

Cat no : 83519-4-RR

Synonyms

SODM, MNSOD, Mn SOD, IPOB, IPO B

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Validation Data Gallery

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  WB analysis using 83519-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83519-4-RR (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 83519-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 83519-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 83519-4-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (83519-4-RR, Clone: 240462H8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HL-60 using 83519-4-RR

  1x10^6 HL-60 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A549 using 83519-4-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83519-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83519-4-RR against Human SOD2 were performed. The affinity constant is 219 nM.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells
• Positive FC (Intra) detected in: A549 cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83519-4-RR is SOD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SOD2 fusion protein Ag21388
• Full Name: superoxide dismutase 2, mitochondrial
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC016934
• Gene Symbol: SOD2
• Gene ID (NCBI): 6648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.