SOD2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SOD2 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A6C2

Cat no : 66474-1-PBS

Synonyms

IPO B, Mn SOD, MNSOD, SOD2, SODM

---

Validation Data Gallery

• 

  WB analysis using 66474-1-Ig (same clone as 66474-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66474-1-Ig (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66474-1-Ig (same clone as 66474-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66474-1-Ig (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66474-1-Ig (same clone as 66474-1-PBS)

  WB result of SOD2 antibody (66474-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD2 transfected HeLa cells. This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66474-1-Ig (same clone as 66474-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66474-1-Ig (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:40000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66474-1-Ig (SOD2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HUVEC using 66474-1-Ig (same clone as 66474-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (66474-1-Ig, Clone: 3A6C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66474-1-Ig (same clone as 66474-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SOD2 (66474-1-Ig, Clone:3A6C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66474-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66474-1-PBS targets SOD2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SOD2 fusion protein Ag21388
• Full Name: superoxide dismutase 2, mitochondrial
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC016934
• Gene Symbol: SOD2
• Gene ID (NCBI): 6648
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.