SOCS6 Polyclonal antibody

SOCS6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27343-1-AP

Synonyms

CIS 4, CIS4, HSPC060, SOCS 4, SOCS 6, SOCS4, SOCS6, SSI4, STAI4, STATI4

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Validation Data Gallery

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  WB analysis using 27343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27343-1-AP (SOCS6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 27343-1-AP

  IP result of anti-SOCS6 (IP:27343-1-AP, 4ug; Detection:27343-1-AP 1:500) with human placenta tissue lysate 1160 ug.

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  IHC staining of human colon using 27343-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27343-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human colon tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27343-1-AP targets SOCS6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOCS6 fusion protein Ag25594
• Full Name: suppressor of cytokine signaling 6
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-75 kDa
• GenBank Accession Number: BC020082
• Gene Symbol: SOCS6
• Gene ID (NCBI): 9306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOCS6 (Suppressor of cytokine signaling 6) is also named as CIS4 and SOCS4. SOCS6 is one of eight members of the SOCS family of proteins. SOCS6 is involved in negative regulation of receptor signaling by increasing degradation mediated by ubiquitination of receptors or substrate proteins and induces apoptosis by targeting mitochondrial proteins (PMID: 25172101). SOCS6 is widely expressed in many tissues and is found to be downregulated in many cancers including colorectal cancer, gastric cancer, lung cancer, ovarian cancer, stomach cancer, thyroid cancer, hepatocellular carcinoma, and pancreatic cancer (PMID: 25172101). In addition to its molecular weight of 60 kDa, SOCS6 has a 70 kDa isoform (PMID: 34169835, PMID: 22125600).