SNX9 Monoclonal antibody, PBS Only

SNX9 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B11C9

Cat no : 68255-1-PBS

Synonyms

MST155, MSTP155, Protein SDP1, SDP1, SH3PX1, SH3PXD3A, SNX9, sorting nexin 9, WISP

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Validation Data Gallery

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  WB analysis using 68255-1-Ig (same clone as 68255-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68255-1-Ig (SNX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68255-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68255-1-Ig (same clone as 68255-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68255-1-Ig (SNX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68255-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68255-1-Ig (same clone as 68255-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using SNX9 antibody (68255-1-Ig, Clone: 1B11C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68255-1-PBS in a different storage buffer formulation.

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Product Information

68255-1-PBS targets SNX9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SNX9 fusion protein Ag8446
• Full Name: sorting nexin 9
• Calculated Molecular Weight: 595 aa, 67 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC005022
• Gene Symbol: SNX9
• Gene ID (NCBI): 51429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).