SNX9 Polyclonal antibody

• Featured Product
• KD/KO Validated

SNX9 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 15721-1-AP

Synonyms

SH3PX1, SH3PXD3A, SH3 and PX domain-containing protein 3A, SH3 and PX domain-containing protein 1, SDP1

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Validation Data Gallery

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  WB analysis using 15721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15721-1-AP (SNX9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15721-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15721-1-AP (SNX9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15721-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15721-1-AP (SNX9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 15721-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15721-1-AP (SNX9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 15721-1-AP

  IP result of anti-SNX9 (IP:15721-1-AP, 3ug; Detection:15721-1-AP 1:1000) with mouse heart tissue lysate 9500ug.

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  IF Staining of HeLa using 15721-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SNX9 antibody (15721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15721-1-AP

  Immunofluorescent analysis of HeLa cells using 15721-1-AP (SNX9 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HeLa cells, rat heart tissue, human heart tissue, mouse skeletal muscle tissue, mouse heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

15721-1-AP targets SNX9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNX9 fusion protein Ag8440
• Full Name: sorting nexin 9
• Calculated Molecular Weight: 595 aa, 67 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC005022
• Gene Symbol: SNX9
• Gene ID (NCBI): 51429
• RRID: AB_2286415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).

Publications

Species	Application	Title
human	WB	Immunity; cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity; Authors - Yunlong Zhao
mouse	WB	Nat Commun; Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs.; Authors - Kiran Todkar ; KD Validated
human	IF	Cancer Discov; In vivo role of INPP4B in tumor and metastasis suppression through regulation of PI3K/AKT signaling at endosomes.; Authors - Chen Li Chew
human	WB	Dev Cell; Mitochondrial-derived vesicles compensate for loss of LC3-mediated mitophagy.; Authors - Christina G Towers ; KD Validated
mouse	WB	Proc Natl Acad Sci U S A; Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease.; Authors - Takahiro Kawasaki
mouse	WB	Oncotarget; Sorting nexin 9 (SNX9) is not essential for development and auditory function in mice.; Authors - Chengcheng Liu ; KO Validated

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