SNX8 Polyclonal antibody

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  WB analysis using 11494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11494-1-AP (SNX8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 11494-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11494-1-AP (SNX8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11494-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11494-1-AP (SNX8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SNX8 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11494-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Mvp1, SNX8, sorting nexin 8

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11494-1-AP targets SNX8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNX8 fusion protein Ag2055
• Full Name: sorting nexin 8
• Calculated Molecular Weight: 465 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC021565
• Gene Symbol: SNX8
• Gene ID (NCBI): 29886
• RRID: AB_2923557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5X2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sorting nexin 8 (SNX8) is a member of the sorting nexin family. Members of the sorting nexin family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX8 plays a role in the endosome-to-Golgi transport (PMID: 19782049). SNX8 can be used as a potential target for treating lysosomal storage disorder (PMID: 38519472).