Various lysates were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10114-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
human placenta tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10114-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10114-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10114-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 10114-1-AP
IP result of anti-SNX6 (IP:10114-1-AP, 4ug; Detection:10114-1-AP 1:600) with HepG2 cells lysate 4000ug.
Immunohistochemical analysis of paraffin-embedded human heart slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate cancer using 10114-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10114-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10114-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNX6 antibody (10114-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNX6 antibody (10114-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of RAW 264.7 using 10114-1-AP
1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug SNX6 Polyclonal antibody (10114-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug SNX6 Polyclonal antibody (10114-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
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HeLa cells, HepG2 cells, human placenta tissue, mouse brain tissue, RAW 264.7 cells
Positive IP detected in
HepG2 cells
Positive IHC detected in
mouse brain tissue, human prostate cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
RAW 264.7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Sorting nexins (SNX) have previously been shown to regulate the cell-surface expression of the human epidermal growth factor receptor. On the basis of the predicted protein sequences, several members of this family, including SNX6, have been identified. SNX6, containing coiled-coil regions within its large C-terminal domain and is found distributed in both membrane and cytosolic fractions, typical of hydrophilic peripheral membrane proteins. The functions of SNX6 are likely to be unique to endosomes, mediated in part by interactions with SNX6-specific C-terminal sequences and membrane-associated determinants
Various lysates were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10114-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10114-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10114-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10114-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 10114-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10114-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10114-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate cancer using 10114-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 10114-1-AP
Immunohistochemical analysis of paraffin-embedded human heart slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 10114-1-AP
Immunohistochemical analysis of paraffin-embedded human heart slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate cancer using 10114-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10114-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HepG2 using 10114-1-AP
IP result of anti-SNX6 (IP:10114-1-AP, 4ug; Detection:10114-1-AP 1:600) with HepG2 cells lysate 4000ug.
IF/ICC Figures
IF Staining of HepG2 using 10114-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNX6 antibody (10114-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of RAW 264.7 using 10114-1-AP
1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug SNX6 Polyclonal antibody (10114-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
SNX6 Polyclonal antibody
Catalog Number
10114-1-AP
Citations
3
Dilutions
WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
