SNX32 Polyclonal antibody

• Featured Product
• KD/KO Validated

SNX32 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25763-1-AP

Synonyms

SNX32, SNX6B, sorting nexin 32, Sorting nexin 6B

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Validation Data Gallery

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  WB analysis of HeLa using 25763-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25763-1-AP (SNX32 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25763-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25763-1-AP (SNX32 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of mouse brain using 25763-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25763-1-AP (SNX32 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25763-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25763-1-AP (SNX32 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

25763-1-AP targets SNX32 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNX32 fusion protein Ag22653
• Full Name: sorting nexin 32
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC040981
• Gene Symbol: SNX32
• Gene ID (NCBI): 254122
• RRID: AB_2880229
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX32 (Sorting nexin-32, also known as SNX6B) may be involved in several stages of intracellular trafficking.

Publications

Species	Application	Title
human,pig	CoIP,WB,IF,IP	J Virol; SNX32 is a host restriction factor that degrades African swine fever virus CP204L via the RAB1B-dependent autophagy pathway; Authors - Wenping Yang ; KD Validated