SNX18 Polyclonal antibody

SNX18 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21946-1-AP

Synonyms

SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18

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Validation Data Gallery

• 

  WB analysis of HL-60 using 21946-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 21946-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21946-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21946-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HL-60 cells, human placenta tissue, L02 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21946-1-AP is SNX18 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNX18 fusion protein Ag16645
• Full Name: sorting nexin 18
• Calculated Molecular Weight: 628 aa, 69 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC060791
• Gene Symbol: SNX18
• Gene ID (NCBI): 112574
• RRID: AB_11182387
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).