SNX10 Polyclonal antibody

SNX10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26727-1-AP

Synonyms

Sorting nexin-10, sorting nexin 10

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Validation Data Gallery

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  WB analysis using 26727-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26727-1-AP (SNX10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 26727-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26727-1-AP (SNX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 26727-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26727-1-AP (SNX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26727-1-AP targets SNX10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNX10 fusion protein Ag24977
• Full Name: sorting nexin 10
• Calculated Molecular Weight: 201 aa, 24 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC034992
• Gene Symbol: SNX10
• Gene ID (NCBI): 29887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins classified by the presence of a phospholipid-binding motif PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. Mutations in SNX10 (Sorting nexin-10) have been found to account for approximately 4% of all human autosomal recessive osteopetrosis (ARO) that is a genetically heterogeneous disorder caused by reduced bone resorption by osteoclasts (PMID: 23280965; PMID: 28592808).