SNUPN Polyclonal antibody

SNUPN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15358-1-AP

Synonyms

Snurportin-1, Snurportin1, snurportin 1, RNUT1, RNA U transporter 1

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Validation Data Gallery

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  WB analysis of HeLa using 15358-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15358-1-AP (SNUPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15358-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15358-1-AP (SNUPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 15358-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15358-1-AP (SNUPN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15358-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15358-1-AP (SNUPN antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 15358-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SNUPN antibody 15358-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa cells using 15358-1-AP

  IF result of 15358-1-AP from Dr. Ralph Kehlenbach.

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  IF Staining of HeLa using 15358-1-AP

  Immunofluorescent analysis of HeLa cells using 15358-1-AP (SNUPN antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

15358-1-AP targets SNUPN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNUPN fusion protein Ag7604
• Full Name: snurportin 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC004203
• Gene Symbol: SNUPN
• Gene ID (NCBI): 10073
• RRID: AB_2192073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; SNUPN deficiency causes a recessive muscular dystrophy due to RNA mis-splicing and ECM dysregulation; Authors - Marwan Nashabat
human	WB	Neurobiol Dis; UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.; Authors - Simona Rossi