RCC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

RCC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10697-1-AP

Synonyms

RCC1, SNHG3 RCC1, SNHG3-RCC1

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Validation Data Gallery

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  WB analysis of HEK-293 using 10697-1-AP

  WB result of RCC1 antibody (10697-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCC1 transfected HEK-293 cells.

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  WB analysis using 10697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10697-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10697-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10697-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 10697-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10697-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 10697-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10697-1-AP

  IP result of anti-RCC1 (IP:10697-1-AP, 4ug; Detection:10697-1-AP 1:500) with HeLa cells lysate 1600 ug.

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  IHC staining of human ovary tumor using 10697-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10697-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10697-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10697-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells, mouse testis tissue, Raji cells, SGC-7901 cells, HeLa cells, HEK-293 cells, HEK-293T cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10697-1-AP targets RCC1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RCC1 fusion protein Ag1042
• Full Name: regulator of chromosome condensation 1
• Calculated Molecular Weight: 45 kDa, 48 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC007300
• Gene Symbol: RCC1
• Gene ID (NCBI): 1104
• RRID: AB_513962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.