SNF8 Monoclonal antibody, PBS Only

SNF8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C10F2

Cat no : 67696-1-PBS

Synonyms

Dot3, EAP30, ESCRT II complex subunit VPS22, hVps22, SNF8, Vacuolar sorting protein SNF8, VPS22

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Validation Data Gallery

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  WB analysis using 67696-1-Ig (same clone as 67696-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNF8 antibody (67696-1-Ig, Clone: 2C10F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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Product Information

67696-1-PBS targets SNF8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SNF8 fusion protein Ag30384
• Full Name: SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
• Calculated Molecular Weight: 258 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC008976
• Gene Symbol: SNF8
• Gene ID (NCBI): 11267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.