SNCG Polyclonal antibody

SNCG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16398-1-AP

Synonyms

Gamma synuclein, Persyn, PRSN, SNCG, SR, Synoretin

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Validation Data Gallery

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  WB analysis using 16398-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16398-1-AP (SNCG antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 16398-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16398-1-AP (SNCG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16398-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16398-1-AP (SNCG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-29 cells, HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16398-1-AP targets SNCG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNCG fusion protein Ag9493
• Full Name: synuclein, gamma (breast cancer-specific protein 1)
• Calculated Molecular Weight: 127 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC014098
• Gene Symbol: SNCG
• Gene ID (NCBI): 6623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gamma synuclein, also known as SNCG, BCSG1, belongs to the synuclein family. SNCG is a synuclein protein found primarily in the peripheral nervous system and retina(PMID 11806835). SNCG is the least conserved of the synuclein proteins and is detected in the brain, ovarian tumors, and the olfactory epithelium(PMID 9750188). SNCG is expressed in high abundance in breast cancer but scarcely at all in a normal breast and is considered a breast cancer marker(PMID: 10813729).