SNCB Polyclonal antibody

SNCB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10498-1-AP

Synonyms

Beta-synuclein, Beta synuclein

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 10498-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10498-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10498-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10498-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10498-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10498-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10498-1-AP

  IP result of anti-SNCB (IP:10498-1-AP, 4ug; Detection:10498-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 10498-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10498-1-AP (SNCB antibody) at dilution of 1:10 (under 10x lens).

• 

  IF Staining of HepG2 using 10498-1-AP

  Immunofluorescent analysis of HepG2 cells, using SNCB antibody 10498-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:10-1:100
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10498-1-AP targets SNCB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNCB fusion protein Ag0772
• Full Name: synuclein, beta
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14-18 kDa
• GenBank Accession Number: BC002902
• Gene Symbol: SNCB
• Gene ID (NCBI): 6620
• RRID: AB_2270787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.