mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10498-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10498-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10498-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10498-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10498-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10498-1-AP
IP result of anti-SNCB (IP:10498-1-AP, 4ug; Detection:10498-1-AP 1:500) with mouse brain tissue lysate 4000ug.
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mouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected in
mouse brain tissue
Positive IHC detected in
human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:10-1:100
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
10498-1-AP targets SNCB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10498-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10498-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10498-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10498-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10498-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 10498-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10498-1-AP (SNCB antibody) at dilution of 1:10 (under 10x lens).
IP Figures
IP experiment of mouse brain using 10498-1-AP
IP result of anti-SNCB (IP:10498-1-AP, 4ug; Detection:10498-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IF/ICC Figures
IF Staining of HepG2 using 10498-1-AP
Immunofluorescent analysis of HepG2 cells, using SNCB antibody 10498-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SNCB Polyclonal antibody
Catalog Number
10498-1-AP
Citations
-
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:10-1:100 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
