SNAP25 Recombinant antibody

SNAP25 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240069F12

Cat no : 83259-5-RR

Synonyms

SNAP 25, SNAP, RIC-4, RIC4, RIC 4

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Validation Data Gallery

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  WB analysis using 83259-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83259-5-RR (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 83259-5-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SNAP25 antibody (83259-5-RR, Clone: 240069F12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of PC-12 using 83259-5-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using SNAP25 antibody (83259-5-RR, Clone: 240069F12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  Affinity and Kinetic Characterization of 83259-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83259-5-RR against Human SNAP25 were performed. The affinity constant is 0.388 nM.

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Tested Applications

• Positive WB detected in: PC-12 cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: PC-12 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83259-5-RR is SNAP25 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SNAP25 fusion protein Ag6695
• Full Name: synaptosomal-associated protein, 25kDa
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC010647
• Gene Symbol: SNAP25
• Gene ID (NCBI): 6616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.