SNAP25 Recombinant antibody, PBS Only (Detector)

SNAP25 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240069A1

Cat no : 83259-1-PBS

Synonyms

SNAP 25, SNAP, RIC-4, RIC4, RIC 4

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Validation Data Gallery

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  IHC staining of mouse brain using 83259-1-RR (same clone as 83259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83259-1-RR (SNAP25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83259-1-RR (same clone as 83259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83259-1-RR (SNAP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 83259-1-RR (same clone as 83259-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using SNAP antibody (83259-1-RR, Clone: 240069A1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-12 using 83259-1-RR (same clone as 83259-1-PBS)

  1x10^6 PC-12 cells were intracellularly stained with 0.25 ug SNAP25 Recombinant antibody (83259-1-RR, Clone:240069A1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00196-1

  Cytometric bead array standard curve of MP00196-1, SNAP25 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83259-3-PBS. Detection antibody: 83259-1-PBS. Standard: Ag6695. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83259-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83259-1-RR against Human SNAP25 were performed. The affinity constant is 4.73 nM.

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Product Information

83259-1-PBS targets SNAP25 as part of a matched antibody pair:

MP00196-1: 83259-3-PBS capture and 83259-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SNAP25 fusion protein Ag6695
• Full Name: synaptosomal-associated protein, 25kDa
• Calculated Molecular Weight: 23 kDa
• GenBank Accession Number: BC010647
• Gene Symbol: SNAP25
• Gene ID (NCBI): 6616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by AX type E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.